CRISPR-Cas9 breaks genes better if you disrupt DNA repair

August 18, 2016

CRISPR-Cas9 is the go-to technique for knocking out genes in human cell lines to discover what the genes do, but the efficiency with which it disables genes can vary immensely. UC Berkeley researchers have now found a way to boost the efficiency with which CRISPR-Cas9 cuts and disables genes up to fivefold, in most types of human cells, making it easler to create and study knockout cell lines and, potentially, disable a mutant gene as a form of human therapy.