Dynamic relocalization of yeast cell cycle regulator, H s l 7

H s l 7 protein, fused to the Green Fluorescent Protein (G F P), is visualized by fluorescence microscopy (upper panels). Hsl7 appears as a "dot" in cells immediately after completion of cell division because it associates with the spindle pole body (the major microtubule-organizing center in yeast cells), situated on the nuclear envelope. Later, however, H s l 7 moves and appears as a "ring" at the bud neck (upper right panel), where it associates with filaments comprised of septin proteins that demarcate the site where cell division will eventually occur. Nuclei in the same cells (lower panels) were visualized by staining with a DNA-specific fluorescent dye (D A P I). This is part of the research in the laboratory of Jeremy Thorner in the Department of Molecular and Cell Biology .

Photo submitted by Jeremy Thorner